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Nitrate/Nitrite Fluorometric Assay Kit

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Cat.No.:
Kit-0629
Product Overview:
Nitrate/Nitrite Fluorometric Assay Kit is a measurement for detecting total nitratr/nitrite concentration.
Description:
Nitric Oxide (NO) is synthesized in biological systems by the enzyme Nitric Oxide Synthase (NOS). NOS is a remarkably complex enzyme which acts on molecular oxygen, arginine, and NADPH to produce NO, citrulline, and NADP+. This process requires five additional cofactors (FMN, FAD, Heme, calmodulin, and tetrahydrobiopterin) and two divalent cations (calcium and heme iron). Three distinct isoforms of NOS have been identified.
Usage:
For research use only (RUO)
Storage:
The kit will perform as specified if components are stored as directed below and used before the expiration date indicated on the outside of the box.Item StorageNitrate/Nitrite Assay Buffer 4°CNitrate Reductase Enzyme Preparation -20°CNitrate Reductase Cofactor Preparation -20°CNitrate Standard 4°C or Room TemperatureNitrite Standard 4°C or Room TemperatureDAN Reagent 4°CSodium Hydroxide (2.8 M) 4°C or Room Temperature96-Well Plate (white) Room Temperature96-Well Cover Sheet Room Temperature
Kit Components:
Nitrate/Nitrite Assay Buffer 1 vialNitrate Reductase Enzyme Preparation 2 vialsNitrate Reductase Cofactor Preparation 2 vialsNitrate Standard 1 vialNitrite Standard 1 vialDAN Reagent 1 vialSodium Hydroxide (2.8 M) 1 vial96-Well Plate (white) 3 plates96-Well Cover Sheet 3 covers


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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