Nuclear/Cytosol Fractionation Kit is used for separating of nuclear extract from the cytoplasmic fraction of mammalian cells.
This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
For research use only (RUO)
Store the kit at -20°C.
Cytosol Extraction Buffer A (CEB-A). Cap code: WM. 5 mlCytosol Extraction Buffer B (CEB-B). Cap code: green. 300 2lNuclear Extraction Buffer A (NEB). Cap code: NM. 2.5 mlDTT (1 M). Cap code: blue. 100 µlProtease Inhibitor Cocktail. Cap code: red. 1 vial
Compatible Sample Types:
Cell Culture, Tissue