Oxaloacetate Assay Kit is a quantitative colorimetric/fluorimetric determination of oxaloacetate at OD570, FL530/585 nm.
OXALOACETATE (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate. Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the oxaloacetate concentration in the sample.
Direct Assays: oxaloacetate in plasma, serum, tissue and culture media.
For research use only (RUO)
Store all kit components at -20 °C. Shelf life of six months after receipt.
Developer 10 mLDye Reagent 120 µLODC Enzyme 120 µLOxaloacetate Standard Dried
Compatible Sample Types:
Culture Medium, Plasma, Serum, Tissue
Features & Benefits:
Sensitive and accurate. Linear detection range: 7 to 400 µM oxaloacetate for colorimetric assays and 1 to 40 µM for fluorimetric assays.