The Oxygen Consumption/Glycolysis Dual Assay Kit is a multi-parameter approach to measure cellular oxygen consumption and glycolysis in living cells. This assay utilizes, a phosphorescent oxygen probe, to measure oxygen consumption rate, and also quantifies extracellular lactate as a readout for glycolysis. Antimycin A, an inhibitor of the mitochondrial electron transport chain, is included as a control. The kit can be used for efficient screening of compounds that modulate mitochondrial and glycolytic function in cultured cells.
MitoXpress - Xtra: 1 vial; 4°CHS Mineral Oil Assay Reagent: 1 vial/15 ml; Room Temperature in the darkCell-Based Assay Antimycin A: 1 vial/200 μl; -20°CGlycolysis Assay Substrate: 1 vial/250 μl; -20°CGlycolysis Assay Enzyme Mixture: 1 vial/lyophilized; -20°CGlycolysis Assay L-Lactate Standard: 2 vials/lyophilized; -20°CGlycolysis Assay Cofactor: 1 vial/250 μl; -20°CCell-Based Assay Buffer Tablet: 1 tablet; Room Temperature