PARP15 (also known as BAL3 or ARTD7) is a transcriptional repressor that exhibits poly(ADP-ribose) polymerase activity.
The PARP15 Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. The key to the PARP15 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP15 reactions. First, histone proteins are coated onto the wells of the strip-plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP15 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
PARP15: 10 µg; -80°C 5x histone mixture: 1 ml; -80°C 10x Assay Mixture Containing Biotinylated Substrate: 300 µl; -80°C 10x PARP assay buffer: 1 ml; -20°C Blocking buffer: 25 ml; +4°C Streptavidin-HRP: 100 µl; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well module plate: 1; Room Temp.