PARP6 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones.
The PARP6 Chemiluminescent Activity Assay kit is designed to measure PARP6 activity for screening and profiling applications. The key to the PARP6 chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP6 reactions. First, histone proteins are coated on a microplate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP6 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
PARP6: 20 µg; -80°C 5x histone mixture: 1 ml; -80°C Opti-PARP 10x assay mixture containing biotinylated substrate: 300 µl; -80°C 10x PARP assay buffer: 1 ml; -20°C Blocking buffer: 25 ml; +4°C Activated DNA (5x): 500 µl; -80°C Streptavidin-HRP: 100 µl; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Max 96-well module plate: 1; Room Temp.