The PD-1:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with the biotinylated PD-L1 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Useful for screening for inhibitors of PD-1 binding to PD-L1
At least one year from date of receipt when stored as directed.
PD-1-FLAG-Avi-His: 30 µg; -80°C PD-L1-biotin: 5 µg; -80°C 3x PD-1 assay buffer: 4 ml; -20°C