Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > PDE2A Assay Kit II

PDE2A Assay Kit II

   Share
"PDE2A" Related Products.
By Species
Human(10)
Mouse(7)
Rat(4)
Rhesus Macaque(1)
By Tag
GST(1)
His(3)
Cat.No.:
Kit-1833
Product Overview:
Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. PDE2A, also known as cAMP-stimulated phosphodiesterase, hydrolyzes cyclic nucleotides cAMP (Km = 2.4M) and cAMP, and is involved in the regulation of blood pressure and fluid homeostasis.
Size:
384 reactions
Description:
The PDE2A Assay Kit is designed for identification of PDE2A inhibitors using fluorescence polarization.The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE2A to the binding agent. PDE2A catalyzes the hydrolysis of the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP). Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. The key to the PDE2A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE2A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE2A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.
Applications:
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Kit Components:
PDE2A1 recombinant enzyme: 1 µg; -80°C FAM-Cyclic-3′, 5′-AMP (20 µM): 50 µl; -80°C PDE assay buffer: 25 ml; -20°C Binding Agent: 250 µl; +4°C cAMP Binding Agent Diluent: 25 ml; +4°C Black, low binding, microtiter plate: 1; Room temp.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.