Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > PDE3B Assay Kit II

PDE3B Assay Kit II

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By Species
Chicken(1)
Human(3)
Mouse(3)
Rat(3)
By Tag
GST(2)
His(2)
Cat.No.:
Kit-1837
Product Overview:
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3B, also known as cGMP-inhibited phosphodiesterase, is involved in mediating the antilipolytic and anti glycogenlytic effects of insulin in adipose and liver tissues. PDE3B can hydrolyze both cAMP and cGMP.
Size:
384 reactions
Description:
The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent. The key to the PDE3B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
Applications:
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage:
At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
PDE3B recombinant enzyme: 1 µg; -80°C FAM-Cyclic-3′, 5′-AMP (20 µM): 50 µl; -80°C PDE assay buffer: 25 ml; -20°C Binding Agent: 250 µl; +4°C Binding Agent Diluent: 25 ml; +4°C Black, low binding, microtiter plate: 1; Room temp.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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