PDE4A Assay Kit
Cat.No. : | Kit-1838 |
Product Overview : | Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4A is widely expressed in brain tumors and promotes their growth and its inhibitor Rolipram has been identified as a potent anti-inflammatory agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. |
- Specification
- Gene Information
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Size : | 96 reactions |
Applications : | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
Storage : | At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Products Types
◆ Recombinant Protein | ||
Pde4a-4745M | Recombinant Mouse Pde4a Protein, Myc/DDK-tagged | +Inquiry |
PDE4A-6584M | Recombinant Mouse PDE4A Protein, His (Fc)-Avi-tagged | +Inquiry |
PDE4A-3990R | Recombinant Rat PDE4A Protein, His (Fc)-Avi-tagged | +Inquiry |
PDE4A-4330R | Recombinant Rat PDE4A Protein | +Inquiry |
PDE4A-29933TH | Recombinant Human PDE4A | +Inquiry |
◆ Lysates | ||
PDE4A-3352HCL | Recombinant Human PDE4A 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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