Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > PDH Activity Assay Kit

PDH Activity Assay Kit

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Cat.No.:
Kit-0669
Size:
96 rxn
Description:
The PDH Activity Assay Kit is used to determine the activity of pyruvate dehydrogenase (PDH) in a sample. The PDH enzyme is immunocaptured within the wells of the microplate and PDH activity is determined by following the reduction of NAD+ to NADH, coupled to the reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm.PDH is the key regulatory enzyme of cellular metabolism because it links the TCA cycle and subsequent oxidative phosphorylation with glycolysis and gluconeogenesis as well as with both lipid and amino acid metabolism. PDH activity is regulated by PDK-dependent phosphorylation and PDP dependent dephosphorylation of PDH. Phosphorylation inactivates PDH whereas dephosphorylation activates PDH.The PDH Activity Assay Kit can be used to elucidate various aspects of PDH activity, physiologic regulation and phosphorylation status.
Storage:
Storage: Multiple storage requirements Do not freeze No Store all components at 4°C. Store the 100X Coupler at -80°C. This kit is Stable for at least 6 months.
Kit Components:
15 ml, 20X Buffer;2 x 1 ml, Detergent;2 x 600 µl, 0X Reagent Mix;1 ea, Microplate;13 ml, 5X Stabilizer;250 µl, 100X Coupler;250 µl, 100X Reagent Dye;This kit contains sufficient materials for 96 measurements (one 96-well microplate).


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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