Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > PDI Inhibitor Screening Assay Kit

PDI Inhibitor Screening Assay Kit

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Cat.No.:
Kit-0671
Product Overview:
PDI Inhibitor Screening Assay Kit provides a simple, homogenous assay for screening modulators of protein disulfide isomerase (PDI) enzymatic activity in a 96-well microplate. The basis for the assay is the PDI-catalyzed reduction of insulin in the presence of DTT, resulting in the formation of insulin aggregates which in turnn bind to the red-emitting fluorogenic PDI Detection Reagent. Relative to analogous turbidimetric assays of PDI activity, the fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit and superior Z"-score (>0.8). The kit includes human recombinant PID enzyme as positive control, as well as the PDI inhibitor bacitracin as a negative control. This kit will provide a quantitative readout of PDI enzymatic activity is a robust and HTP fashion and can be applied to identification of PDI inhibitors from chemical libraries.
Size:
2 x 96 tests
Applications:
Functional Studies more details
Kit Components:
Components: 2 x 96 tests; Deionized Water: 1 x 5ml; DTT: 2 x 1.3ml; Inhibitor Control (Bacitracin): 1 vial; Insulin (from bovine pancreas): 2 vials; PBE Buffer: 1 x 25ml; PDI (Human, Recombinant): 2 x 165µl; PDI Detection Reagent: 1 x 20µl; Stop Reagent: 1 x 1ml
Detection method:
Fluorescent
Compatible Sample Types:
Purified protein, Inhibitor compounds
Sensitivity:
37 µM


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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