Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Activity Kits > PI 3-K Assay Kit

PI 3-K Assay Kit

"Kinase" Related Products.
Product Overview:
A sensitive and robust homogeneous PI3-K assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter.
1 kit/100 Tests
The Calbiochem PI 3-K Assay Kit is a homogeneous assay designed to measure PI 3-K activity using purified enzyme preparations.
Storage: +2°C to +8°C;Storage Conditions: Upon arrival store the entire contents of the kit at 4°C.Do not freeze: No.
Kit Components:
Assay Buffer 3: 1 vial, 10 ml, supplied as 50 mM MgCl₂, 25 mM HEPES, 0.05% sodium azide, pH 7.0Coordination Buffer: 1 vial, 600 µl, supplied as 1X, pH 9.5Sensor Stock: 1 vial, 45 µl, supplied in DMSO/methanol (1:1)Sensor Dilution Buffer: 1 vial, 10 ml, supplied as a MES/NaCl-based buffer, 0.05% sodium azideTrivalent Metal Ion: 1 vial, 10 µl, supplied as 50 mM in 0.5 N HClPost Reaction Buffer: 1 vial (lot-specific volume), supplied as NaCl-based buffer, 0.05% NaN₃
Compatible Sample Types:
Purified enzyme

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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