"Kinase" Related Products

PI 3-K Assay Kit

Cat.No.: Kit-0690
Product Overview: A sensitive and robust homogeneous PI3-K assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter.
Size: 1 kit/100 Tests
Usage: The Calbiochem PI 3-K Assay Kit is a homogeneous assay designed to measure PI 3-K activity using purified enzyme preparations.
Storage: Storage: +2°C to +8°C;Storage Conditions: Upon arrival store the entire contents of the kit at 4°C.Do not freeze: No.
Kit Components: Assay Buffer 3: 1 vial, 10 ml, supplied as 50 mM MgCl₂, 25 mM HEPES, 0.05% sodium azide, pH 7.0Coordination Buffer: 1 vial, 600 µl, supplied as 1X, pH 9.5Sensor Stock: 1 vial, 45 µl, supplied in DMSO/methanol (1:1)Sensor Dilution Buffer: 1 vial, 10 ml, supplied as a MES/NaCl-based buffer, 0.05% sodium azideTrivalent Metal Ion: 1 vial, 10 µl, supplied as 50 mM in 0.5 N HClPost Reaction Buffer: 1 vial (lot-specific volume), supplied as NaCl-based buffer, 0.05% NaN₃
Compatible Sample Types: Purified enzyme

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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