A sensitive and robust homogeneous PI3-K assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter.
The Calbiochem PI 3-K Assay Kit is a homogeneous assay designed to measure PI 3-K activity using purified enzyme preparations.
Storage: +2°C to +8°C;Storage Conditions: Upon arrival store the entire contents of the kit at 4°C.Do not freeze: No.
Assay Buffer 3: 1 vial, 10 ml, supplied as 50 mM MgCl₂, 25 mM HEPES, 0.05% sodium azide, pH 7.0Coordination Buffer: 1 vial, 600 µl, supplied as 1X, pH 9.5Sensor Stock: 1 vial, 45 µl, supplied in DMSO/methanol (1:1)Sensor Dilution Buffer: 1 vial, 10 ml, supplied as a MES/NaCl-based buffer, 0.05% sodium azideTrivalent Metal Ion: 1 vial, 10 µl, supplied as 50 mM in 0.5 N HClPost Reaction Buffer: 1 vial (lot-specific volume), supplied as NaCl-based buffer, 0.05% NaN₃
Compatible Sample Types: