"PLAU" Related Products

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PLAU (Human) Chromogenic Activity Assay Kit (Indirect Assay)

Cat.No.: Kit-0703
Product Overview: PLAU (Human) Chromogenic Activity Assay Kit (Indirect Assay) is developed to determine human PLAU activity in plasma and cell culture supernatants.
Description: Urokinase-type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
Target Species: Human
Usage: For research use only (RUO)
Storage: Upon arrival, immediately store components of the kit at recommended temperatures up to the expiration date.Store Standard, Plasminogen, and Plasmin Substrate at -20°C.Store Microplate and Assay Diluent at 2-8°C.Unused microplate wells may be returned to the pouch and resealed.Opened Assay Diluent may be stored for up to 30 days at 2-8°C.
Kit Components: Microplate: One 96-well polystyrene microplate. 96 (8x12) wellSealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay. 3 slicesAssay Diluent: Ready to use diluent. 30 mLHuman uPA Standard: Human high molecular weight uPA (30 IU, lyophilized). 1 vialHuman plasminogen: Lyophilized. 1 vialPlasmin Substrate: Lyophilized. 2 vials
Compatible Sample Types: Cell Culture Supernatant, Plasma, Serum

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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