Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > PLTP Activity Fluorometric Assay Kit II

PLTP Activity Fluorometric Assay Kit II

"PLTP" Related Products.
By Species
By Source
+Expand -Collapse
By Tag
Product Overview:
PLTP Activity Assay Kit II (Fluorometric) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Phosphlipid Transfer Protein (PLTP). PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 465/535 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum as positive control for assay validation.This assay can measure PLTP activity in serum but can also be used for testing activity of recombinant PLTP protein.
Phospholipid Transfer Protein (PLTP) is a protein found in mammalian plasma that plays an important role in transferring phospholipids between high-density lipoprotein (HDL) molecules and in the modulation of HDL size and composition, as well as in controlling plasma HDL levels. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability. Due to its role in cholesterol transport, PLTP maybe promote atherosclerosis and is, therefore, considered a promising target for pharmacological intervention.
Functional Studies
Target Species:
Reacts with: Rabbit, HumanPredicted to work with: all Mammals
Store kit at 4ºC in the dark immediately upon receipt, except for the PLTP positive control which should be stored at -20ºC. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.Refer to list of materials supplied for storage conditions of individual components. Observe the storage conditions for individual prepared components in section 5.Aliquot components in working volumes before storing at the recommended temperature. Reconstituted components are Stable for 2 months.
Kit Components:
Components 100 testsDonor Molecule 1 x 0.2mlPLTP Acceptor Molecule 1 x 0.5mlPLTP Assay Buffer 1 x 20mlPositive Control 1 x 0.1ml
Detection method:
Compatible Sample Types:
Serum, Plasma, Purified protein

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.