PPARγ Ligand Screening/Characterization Assay Kit

• Cat.No.: Kit-2114
• Product Overview: The PPARγ Ligand Screening Assay Kit provides a single step fluorescence-based assay for screening potential PPARγ-specific ligands. The assay utilizes the ability of potential PPARγ-binding ligands to displace a fluorescent probe, which has a strong affinity for PPARγ Ligand Binding Domain, resulting in loss of fluorescence of the probe. The relative drop in the fluorescence, as a result of competitive binding of PPARγ ligand, can be correlated to the affinity (and hence IC50) of the PPARγ candidate ligand. The PPARγ Ligand Screening Assay Kit is easy to use, faster and more convenient as compared to Fluorescence Polarization and TR-FRET-based screening methods. The assay kit can be used to identify and characterize PPARγ-specific ligands for therapeutic applications.

Specification

• Size: 100 assays
• Applications: Rapid, high-throughput screening of drugs and novel ligands. Development of structure-activity relationship (SAR) models to predict PPARγ/ligand interaction liability of novel compounds.
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the assay.PPARγ Assay Buffer: Bring to room temperature before use. Store at -20°C. Avoid prolonged storage of the PPARγ Assay Buffer at room temperature or 4°C.Human PPARγ: Store at -80°C. Avoid repeated freeze/thaw cycles. Each vial contains enough protein for 50 assays.PPARγ Assay Probe and Ligand Control: Store at -20°C. Bring to room temperature before use.