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PPAR alpha Transcription Factor Assay Kit

Cat.No. : Kit-0715
Product Overview : PPAR alpha Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.
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  • Gene Information
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Size : 96 tests
Applications : Functional Studies more details

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What potential therapeutic applications could arise from targeting PPARs in various metabolic diseases? 02/19/2023

Targeting PPARs offers therapeutic potential in treating diabetes, obesity, and related metabolic conditions.

What is the primary function of PPARs in cellular metabolism? 06/23/2022

PPARs (Peroxisome Proliferator-Activated Receptors) are nuclear receptors that regulate gene expression related to metabolism.

What role do PPARs play in the development of metabolic disorders like diabetes and obesity? 12/27/2020

PPARs are key in managing metabolic diseases, influencing insulin sensitivity and energy balance.

How do genetic variations in PPAR genes affect their function and contribute to disease susceptibility? 11/09/2019

Genetic mutations in PPARs can lead to altered metabolic function, increasing the risk of diabetes and obesity.

How do PPARs influence lipid metabolism and energy homeostasis? 11/18/2017

They play a crucial role in lipid metabolism, regulating the storage and burning of fats.

How do PPARs interact with other nuclear receptors and signaling pathways in the cell? 06/22/2017

PPARs interact with other nuclear receptors and metabolic pathways, coordinating a wide range of metabolic processes.

What is the impact of PPAR modulation on cardiovascular health? 06/12/2017

PPAR modulation affects lipid profiles and inflammation, impacting cardiovascular health.

Customer Reviews (3)

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01/09/2022

    Research progress boosted. Consistency in quality and service.

    05/11/2020

      Expertise shines in every analysis. A vital research resource.

      09/12/2019

        Precision in protein analysis. Trusted for reliable results.

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