PPAR (alpha, delta, gamma) Transcription Factor Assay Kit

• Cat.No.: Kit-0714
• Product Overview: PPAR Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR alpha, delta, or gamma are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The kit comes with a single plate that measures all three isoforms of PPAR alpha, delta, and gamma. There are enough reagents for one-third of a plate for each isoform. This product is being discontinued. You can use PPAR alpha Transcription Factor Assay Kit, PPAR delta Transcription Factor Assay Kit or PPAR gamma Transcription Factor Assay Kit as alternative products.

Specification

• Size: 96 tests
• Applications: Functional Studies more details