|Product Overview:||PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.|
|Description:||The PRMT1 Homogeneous Assay Kit is designed to measure the activity of PRMT1 for screening and profiling applications, using AlphaLISA technology. The key to the PRMT1 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT1 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.|
|Applications:||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.|
|Storage:||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.|
|Kit Components:||PRMT1: 1 µg; -80°C 20 µM S-adenosylmethionine: 500 μl; -80°CPrimary antibody 4: 400 μl; -80°CBiotinylated histone H4 peptide substrate: 400 μl; -80°C 4x HMT assay Buffer 2: 3 ml; -20°C 4x Detection buffer: 2 ml; -20°C|
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