"PRMT3" Related Products

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PRMT3 Chemiluminescent Assay Kit

Cat.No.: Kit-1855
Product Overview: PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Size: 96 reactions
Description: The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3 activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: PRMT3 human recombinant enzyme: 10 µg; -80°C 20 µM S-adenosylmethionine*: 250 µl; -80°C Primary antibody 4: 100 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C4x HMT assay buffer 2: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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