PRSS7 Activity Assay Kit (Fluorometric) is used for measuring enteropeptidase/enterokinase activity using fluorometric methods.
Enteropeptidase (Enterokinase, EC 126.96.36.199) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence "DDDDK" and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In PRSS7 Activity Assay Kit (Fluorometric), we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label "AFC". Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU.
Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity.Removing tag from recombinant proteins having recognition motif.
For research use only (RUO)
Store kit at –20°C, protected from light. Warm Assay Buffer, Enteropeptidase Substrate & AFC Standard to room temperature before use. Briefly centrifuge small vials at low speed (high speed not ideal for enzymes) prior to opening. Read the entire protocol before performing the experiment.
Enteropeptidase Assay Buffer 20 mlEnteropeptidase Substrate (10 mM, in DMSO) 0.2 mlHuman Enteropeptidase (Positive Control) 17 μlAFC Standard (1 mM) 100 μl
Compatible Sample Types: