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Home > Products > Assay Kits > Epigenetic Kits > Pan-Methyl Histone H3-K79 Quantification Kit (Colorimetric)

Pan-Methyl Histone H3-K79 Quantification Kit (Colorimetric)

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Product Overview:
Pan-Methyl Histone H3-K79 Quantification Kit (Colorimetric) is use for measuring mono, di-and tri-methylation of histone H3-K79.
Epigenetic activation or inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. Dot1 is a histone methyltransferase that catalyzes methylation of histone H3 at lysine 79 (H3-K79) in mammalian cells. Intergenic H3-K79 mono-methylation is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fiber to stabilize interactions between enhancers and their target promoters. H3-K79 diand tri-methylation is also a widespread histone modification and is associated with transcriptionally active genes. Increased H3-K79 methylation is found to be involved in some pathological processes such as leukemogenesis in human. The patterns of H3-K79 methylation can also be changed by inhibition or activation of HMTs. Thus quantitative detection of mono-, di- and tri-methyl histone H3-K79 would provide useful information for better understanding epigenetic regulation of gene activation and for developing HMT targeted drugs. The Pan-Methyl Histone H3-K79 Quantification Kit (Colorimetric) provides a tool for measuring mono, di- and tri-methylation of histone H3-K79.
For simultaneously measuring histone H3-K79 mono-, di-, and tri-methylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
For research use only (RUO)
Upon receipt, store C3 and Standard control at –20°C. Store all other components at 4°C away from light. The components of the kit should be stable for 6 months when stored properly.Note: Check if buffers C1 and C2 contain salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are redissolved.
Kit Components:
C1 (10X wash buffer) 20 mlC2 (antibody buffer) 12 mlC3 (detection antibody, 1 mg/ml)* 10 µlC4 (color developer) 10 µlC5 (stop solution) 6 mlStandard control (100 µg/ml)* 20 µl8 well sample strips (with frame) 98 well standard control strips* 3* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
Features & Benefits:
Quick and efficient procedure, which can be finished within 2.5 hours.Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.Simultaneous quantification of mono-, di-, and tri-methylated H3-K79 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.The control is conveniently included for quantification of the amount of mono-, di– and tri-methylated H3-K79.Strip microplate format makes the assay flexible: manual or high throughput.Simple, reliable, and consistent assay conditions.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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