Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Phenylalanine Assay Kit

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Cat.No.:
Kit-2080
Product Overview:
L-Phenylalanine Assay Kit provides a convenient fluorimetric means to measure L-phenylalanine in biological samples. In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. The resulting fluorescence intensity (λexc/em = 530/585 nm) is linear to the L-phenylalanine concentration in the sample.
Size:
100 tests
Description:
L-Phenylalanine is one of the twenty common amino acids and an important precursor for several key signal molecules such as dopamine, norepinephrine, epinephrine, and the skin pigment melanin. It is found naturally in the breast milk of mammals, and used as nutritional supplements in food and drink products. The genetic disorder phenylketonuria is the inability to metabolize phenylalanine. Individuals with this disorder are known as "phenylketonurics". Individuals who cannot metabolize phenylalanine must monitor their intake of protein to control the buildup of phenylalanine.
Applications:
For quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism.
Storage:
The kit is shipped on ice. Store all reagents at -20°C except for reconstituted Enzyme A. Shelf life of 6 months after receipt.
Kit Components:
Assay Buffer: 10 mL Enzyme A: DriedNAD Solution: 1 mL Enzyme B: 120 µLProbe: 750 µL Standard: 120 µL
Detection method:
FL530/595nm
Compatible Sample Types:
Serum, urine etc
Features & Benefits:
• Safe. Non-radioactive assay.• Sensitive and accurate. Linear detection range of 2 - 300 μM L-phenylalanine.• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Assay time:
30 min
Sensitivity:
Detection Limit: 2 μM


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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