L-Phenylalanine Assay Kit provides a convenient fluorimetric means to measure L-phenylalanine in biological samples. In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. The resulting fluorescence intensity (λexc/em = 530/585 nm) is linear to the L-phenylalanine concentration in the sample.
L-Phenylalanine is one of the twenty common amino acids and an important precursor for several key signal molecules such as dopamine, norepinephrine, epinephrine, and the skin pigment melanin. It is found naturally in the breast milk of mammals, and used as nutritional supplements in food and drink products. The genetic disorder phenylketonuria is the inability to metabolize phenylalanine. Individuals with this disorder are known as "phenylketonurics". Individuals who cannot metabolize phenylalanine must monitor their intake of protein to control the buildup of phenylalanine.
For quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism.
The kit is shipped on ice. Store all reagents at -20°C except for reconstituted Enzyme A. Shelf life of 6 months after receipt.
Assay Buffer: 10 mL Enzyme A: DriedNAD Solution: 1 mL Enzyme B: 120 µLProbe: 750 µL Standard: 120 µL
Compatible Sample Types:
Serum, urine etc
Features & Benefits:
• Safe. Non-radioactive assay.• Sensitive and accurate. Linear detection range of 2 - 300 μM L-phenylalanine.• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Limit: 2 μM