Phosphatidylcholine Assay Kit is used for measuring phosphatidylcholine.
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. The Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em = 535/587 nm) and absorbance (570 nm). The kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).
For research use only (RUO)
Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.
PC Assay Buffer 25 mLOxiRed Probe 0.2 mLPC Hydrolysis Enzyme, lyophilize. 1 vialPC Development Mix, lyophilize. 1 vialPC Standard (10 μmol), lyophilize. 1 vial
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine