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Phosphatidylcholine Assay Kit

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GST(1)
His(4)
Cat.No.:
Kit-0662
Product Overview:
Phosphatidylcholine Assay Kit is used for measuring phosphatidylcholine.
Description:
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. The Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em = 535/587 nm) and absorbance (570 nm). The kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.
Kit Components:
PC Assay Buffer 25 mLOxiRed Probe 0.2 mLPC Hydrolysis Enzyme, lyophilize. 1 vialPC Development Mix, lyophilize. 1 vialPC Standard (10 μmol), lyophilize. 1 vial
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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