Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Metabolism Kits > Phosphoenolpyruvate Assay Kit

Phosphoenolpyruvate Assay Kit

"PEP" Related Products.
Product Overview:
Phosphoenolpyruvate Assay Kit is a quantitative colormetric/fluorometric determination of phosphoenolpyruvate.
Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco- neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system used acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase. Phosphoenolpyruvate Assay Kit provides a convenient colorimetric and fluorometric means to measure PEP levels. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em 535/587 nm). The assay is simple, sensitive and stable. The detection limit is approximately 1 µM PEP in biological samples.
Target Species:
For research use only (RUO)
Store kit at –20°C, protect from light. Warm the PEP Assay Buffer to room temperature prior to use. Briefly centrifuge all vials prior to opening. Read the entire protocol before performing the assay. Not included but needed: liquid N2 or dry/ice methanol, 3M HClO4, 3M KHCO3, activated charcoal.
Kit Components:
PEP Assay Buffer. Cap code: WM. 25 mlPEP Probe (in DMSO). Cap code: red. 0.2 mlPEP Converter. Cap code: purple. 1 vialPEP Developer Mix. Cap code: green. 1 vialPEP Standard (1 9mole). Cap code: yellow. 1 vial
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Cell Lysate, Plasma, Plant Extract, Serum, Tissue Lysate, Urine, Plant Extract

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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