Phospholipase D Assay Kit is a quantitative colorimetric/fluorimetric phospholipase D activity determination.
PHOSPHOLIPASE D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers.
Direct Assays: phospholipase D in biological samples.Drug Discovery/Pharmacology: effects of drugs on phospholipase D metabolism.
For research use only (RUO)
Store all components at -20°C. Shelf life of six months after receipt.
Assay Buffer 10 mLDye Reagent 120 µLEnzyme Mix (Dried) 1 vialSubstrate 1.5 mLCalibrator 400 µL
Compatible Sample Types:
Features & Benefits:
Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.