Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Plant Tissue Extraction Kit

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Cat.No.:
Kit-2160
Product Overview:
Plant Protein Extraction Kit provides an effective way of extracting soluble protein and other biomolecules and metabolites from all kinds of dry and fresh plant tissues without the use of liquid nitrogen. The kit includes optimized Extraction Buffer to extract plant protein and metabolites, Protease Inhibitor Cocktail to prevent protein degradation and Homogenization Pestles and Tubes to homogenize the plant tissue. The extracted protein can be used in a variety of downstream applications such as Western Blotting, 2-D gel electrophoresis, immunoprecipitation assay, and enzymatic activity assays etc. and extracted small molecules can be used in plant metabolic studies. This easy-to-use and rapid procedure ensures consistent protein yield ~ 2-9 mg/ml depending on the sample type.
Size:
50 assays
Applications:
Extraction of plant soluble protein and metabolites for various downstream applications such as Western Blotting, 2-D gel electrophoresis, immunoprecipitation assay, enzymatic activity assay and quantitation of metabolites etc.
Storage:
Store kit at -20°C, protect from light. Homogenization Pestles and tubes can be stored at room temperature or -20°C. Thaw reagentsbefore use. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.
Kit Components:
Plant Extraction Buffer: 25 mlProtease Inhibitor Cocktail (200X): 1 mlHomogenization Pestles: 3Homogenization Tubes (1.5 ml): 3
Detection method:
Protein Quantitation
Compatible Sample Types:
Fresh or frozen plant tissues, dry plant tissues such as seeds
Features & Benefits:
• Simple method for extracting soluble protein, biomolecules & metabolites from plant tissues without the use of liquid nitrogen• Easy-to-use and rapid procedure ensures consistent protein yield ~ 2-9 mg/ml depending on the sample type


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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