Creative BioMart offers Human and Mouse Cell line lysate which are Ideal for Western blotting. All the lysates are prepared by homogenization in modified RIPA buffer. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Creative BioMart Cell line lysate list