"Proteasome" Related Products

Proteasome 20S assay Kit

Cat.No.: Kit-0723
Size: 96 wells
Description: Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity. A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes.
Applications: Fluorescence microscopy, HTS
Storage: -20°C
Kit Components: Proteasome 20S (human) (purified)Suc-Leu-Leu-Val-Tyr-AMCProteasome Assay BufferSodium Dodecyl SulfateEpoxomicinAMC (7-Amino-4-methylcoumarin) Calibration Standard1/2 Volume White Microplates

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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