Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Protein Creatinine Ratio Assay Kit

Protein Creatinine Ratio Assay Kit

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Cat.No.:
Kit-2082
Product Overview:
PROTEIN/CREATININE RATIO (PCR) remains the simplest and most convenient test for proteinuria. Other methods such as 24 hour urine test or timed urine test require strict adherence to sample collection protocol. Since the protein concentration is normalized to creatinine secretion, the urine sample can be taken at anytime and no diet or liquid restrictions are necessary for sample collection.
Size:
100 tests
Description:
PROTEIN is filtered out of urine by the glomeruli of the kidneys. Albumin is the most common serum protein, thus the majority of the protein in urine is albumin. A damaged kidney will allow some protein through into the urine, the less protein in urine the better. Elevated protein levels in urine is called microalbuminuria or proteinuria, which typically arises due to type 1 diabetes, type 2 diabetes, or high blood pressure. CREATININE is synthesized in the body at a fairly constant rate from creatine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate.
Applications:
Direct Assays: Protein creatinine ratio determination in urine samples (rat, mouse, human, not species specific).Drug Discovery/Pharmacology: effects of drugs on protein and creatinine concentration, metabolism, and excretion.
Storage:
The kit is shipped at room temperature. Store all reagents at 2-8°C. Shelf life: 12 months after receipt.
Kit Components:
PR Reagent: 24 mL CR Reagent A: 6 mLCR Reagent B: 6 mL Standard: 1 mL
Detection method:
OD600 and OD530nm
Compatible Sample Types:
Urine samples
Features & Benefits:
• Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 – 150 mg/dL Creatinine.• Fast and convenient. No sample pre-treatment is needed. Simple 10- minute "add-incubate-read" procedure. • High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day.
Assay time:
10 min
Sensitivity:
Detection Limit: 1 mg/dL Protein and 1 mg/dL Creatinine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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