Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
Inquiry now !
* E-mail Address:
* Service & Products Interested:
Project Description:
* Verification code:
Please input "biomart" as verification code.
Submit

SERVICES
Your Position:  Home >> Services >>Protein Design for Protein Engineering

Protein Design for Protein Engineering

Share

Based upon our protein engineering expertise, we can modulate and optimize challenging protein properties such as activity, affinity, selectivity, stability, resistance to proteolytic degradation, and even improve protein expression and solubility.

Protein design refers to the ability to alter protein structure to achieve the desired protein function. In order to increase the solubility, stability or ability to crystallize of a given protein, we can use a protein compaction technology based on the deletion of the protein’s non-essential floppy loops. Of the two classes of proteins (binding proteins and catalytic proteins), catalytic proteins, or so-called enzymes, are of particular importance to chemical processing because they can be used as chemical catalysts.

Many successful examples using rational design to engineer enzyme stability or activity have been reported. One of them is the above-mentioned redesign of a T4 lysozyme with increased stability by engineering disulfide bonds into the protein. Another notable example is the design of a subtilisin BPN’ mutant containing six site-directed mutations, which was 100-fold more stable than the wild-type enzyme in an aqueous environment and 50-fold more stable than the wild-type in anhydrous dimethylformamide.

This approach has been applied to both viral antigens for vaccines development and anti-bacterial targets for drug discovery applications. The resulting functional mini-protein will display a better therapeutic value (therapeutic proteins, antigens) or a superior ability to crystallize (target proteins).

Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information.
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.