Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Protein Synthesis Assay Kit

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Cat.No.:
Kit-2011
Size:
96 wells
Description:
The Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating polypeptide chains, thereby stopping translation. These truncated C-terminal alkyne-labeled proteins are then subsequently detected via copper-catalyzed click chemistry using 5 FAM-Azide. The reagents provided in the kit are sufficient to run 20 samples when using flow cytometry or 100 samples when using a 96-well plate format.
Storage:
-20°C
Kit Components:
Cell-Based Assay Wash Buffer: 2 vials/100 ml; Room TemperatureCell-Based Assay TBS (10X): 1 vial/10 ml; Room TemperatureO-Propargyl-Puromycin Stock Solution: 1 vial/25 µl; -20°CCopper Sulfate Solution: 1 vial/100 µl; -20°CCell-Based Assay Fixative: 1 vial/10 ml; Room TemperatureAscorbic Acid Solution: 1 vial/2 ml; -20°CCell-Based Assay 5 FAM-Azide Stock Solution: 1 vial/25 µl; -20°CCell-Based Assay Cycloheximide: 1 vial/50 µl; -20°C


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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