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Purine Nucleoside Phosphorylase Activity Fluorometric Assay Kit

Cat.No.: Kit-0712
Product Overview: Rapid, simple & convenient
Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase
Description: Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo-or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T-cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase.
Applications: Detection of Purine Nucleoside Phosphorylase activity in variety of samples
Usage: For Research Use Only! Not For Use in Humans.
Storage: -20°C
Size: 100 assays
Kit Components: • PNP Assay Buffer (10x)
• Enzyme Mix
• Inosine Substrate
• PNP Probe (in dry DMSO)
• Hypoxanthine Standard (10 mM)
• PNP Positive Control
Detection method: Fluorescence (Ex/Em = 535/587 nm)
Compatible Sample Types: • Purified recombinant protein
• Cell and tissue lysate

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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