Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > Pyruvate Kinase Assay Kit
"PK" Related Products.
Product Overview:
Pyruvate Kinase Assay Kit is used for measuring pyruvate kinase activity.
Pyruvate kinase (PK, EC is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. We provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU pyruvate kinase.
Target Species:
For research use only (RUO)
Store the kit at -20°C.
Kit Components:
PK Assay Buffer 25 mlOxiRed Probe 200 µlPK Enzyme Mix LyophilizedPK Substrate Mix LyophilizedPK Positive Control LyophilizedPyruvate Standard (100 nmol/µl) 100 µl
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Food Sample, Growth Medium, Plasma, Serum, Tissue Culture Supernatant, Urine

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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