The panel of RAR reporter assays utilizes non-human mammalian cells engineered to individually express human retinoic acid receptors, RARα (NR1B1), RARβ (NR1B2), or RARγ (NR1B3). The RAR reporter cells include the luciferase reporter gene functionally linked to a responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in RARα, RARβ or RARγ activity. The principal application of this assay panel is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against the three human RAR"s. RAR reporter cells are prepared using the proprietary CryoMite process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. The nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to RAR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user"s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
▪ RARα Reporter Cells: 1 x 0.60 mL; -80°C▪ RARβ Reporter Cells: 1 x 0.60 mL; -80°C▪ RARγ Reporter Cells: 1 x 0.60 mL; -80°C▪ Cell Recovery Media (CRM): 1 x 10.5 mL; -20°C▪ Compound Screening Media (CSM): 1 x 35 mL; -20°C▪ RARα reference agonist: 9 cis-Retinoic Acid, 10 mM: 1 x 30 µL; -20°C▪ RARβ and RARγ reference agonist: trans-Retinoic Acid, 10 mM: 1 x 30 µL; -20°C▪ Detection Substrate: 3 x 2.0 mL; -80°C ▪ Detection Buffer: 3 x 2.0 mL; -20°C▪ 96-well format plate frame: 1; ambient ▪ snap-in, 8-well strips 12 ambient (white, sterile, cell culture treated)