Rac Activation Kit

• Cat.No.: Kit-0757
• Product Overview: Rac Activation Kit provides a rapid, cost-effective and reliable tool for detection and semi-quantitative analysis of the cellular activation state of Rac1 and Rac2 G-proteins. If combined with immunological detection of Cdc42, the kit can also detect activated Cdc42-GTP. Please note that this kit provides only an antibody for the detection of Rac1. Active Rac, i. e. Rac complexed to GTP, is purified out of cell extracts via a one-step batch affinity purification step employing GST-PAK-CRIB, an affinity probe that selectively interacts with the active, GTP-bound Rac conformation in loss of the inactive Rac-GDP complex. The kit further includes recombinant Rac1δC-term that serves dual roles: 1. It can be used as western blot control for immunodetection2. Upon loading with GDP or GTP-γ-S (also included in this kit), Rac1δC-GDP or Rac1DC-GTP-γ-S complexes can be used to "spike" cell extracts thus serving as internal assay controls of signal specificity.

Specification

• Size: 1 kit
• Applications: Functional Studies more details