Recombinant Aequorea victoria GFP (AAB65663) (Met1-Leu238, except for the point mutations of intellectual property?:F64L, S65T, R80K, S99F, T153M, I167V, H231L) was expressed, with a polyhistide tag at the C-terminus (Patent 201410599023.9).
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
GFPSparkTM expression vector transfected 293H cells transiently. After 48h, the strong green fluorescent signals can be detected under the excitation channel of 455~495 nm.
The recombinant Aequorea victoria GFPSparkTM consisting of 245 amino acids and has a calculated molecular mass of 27.7 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.