Recombinant Alternaria alternata ALTA1 was expressed in E. coli.
Alt a 1 is a lyophilized, recombinant protein with IgE-binding capacity. It was produced by heterologous expression in E. coli, purified by conventional biochemical methods.
The lyophilized product can be kept at room temperature for at least 2 weeks. However, we recommend the product to be stored at -20 centigrade. Under these conditions the quality of the material will be maintained for several years. The stability at 4 centigrade should at least be 6 months. Reconstituted protein can be stored at -20 centigrade.
If reconstituted with water or diluted buffer to a concentration of 1 mg/ml, the PO4 buffer concentration will be 2 mM (pH 7.4). Addition of 1-2 mM -Mercaptoethanole is recommended. If reconstituted to a concentration of 2mg/ml, the product is soluble to more than 95%.