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Recombinant Alternaria alternata ENO protein

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Cat.No.:
ENO-563A
Product Overview:
Recombinant Alternaria alternata ENO was expressed in E. coli.
Description:
Alt a 2 (Alt a 6 according to the effective allergen nomenclature) is a lyophilized, recombinant protein with IgE-binding capacity. It was produced by heterologous expression in E. coli, purified by conventional biochemical methods.
Source:
E. coli
Species:
Alternaria alternata
Molecular Mass:
~47,382 Dalton
Purity:
> 98%
Notes:
Enolase activity can be restored by dissolving the lyophilized protein to a concentration of 1 mg/ml in 4.5 M urea and dialyzing it over night against 10mM NaxHxPO4 pH 7.5, containing 2mM -Mercaptoethanole, and 2mM Mg2+.No enzymatic activity was observed after reconstitution in water/ -Mercaptoethanole.
Storage:
When stored at -20 centigrade the quality of the material will be maintained for several years. However, for short periods (max. 3 weeks) the lyophilized product may be kept at room temperature. After reconstitution store at -20 centigrade. Avoid repeated freezing/thawing.
Concentration:
1 mg/ml
Reconstitution:
The material can be reconstituted with distilled water (or equivalent) or dilute buffers. Addition of 1-2 mM -Mercaptoethanol is recommended. If reconstituted to a concentration of 1 mg/ml, the phosphate buffer concentration will be 2 mM (pH 7,4). The product is soluble to more than 95% at a concentration of 2 mg/ml.
UniProt ID:
Q9HDT3


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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