Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Anthrax Protective antigen protein, FITC conjugated

Recombinant Anthrax Protective antigen protein, FITC conjugated

   Share
"Protective antigen" Related Products.
By Species
By Source
Cat.No.:
PA-137
Product Overview:
Anthrax Protective antigen(PA 63) conjugated with FITC was expressed in B. anthracis.
Description:
FITC is prepared by conjugating anthrax protective antigen to fluorescein isothiocyanate, isomer I (FITC) by a modification of the method of Wood et al. PA has been trypsin activated and the PA63 conjugate to FITC has been purified.
Source:
B. anthracis
Species:
B. anthracis
Form:
When reconstituted with 0.2 ml of sterile water, each vial contains 50 ug of protein in 10 mM bis-Tris propane, pH 8.5 with 1.25% trehalose.
Molecular Mass:
When examined on 4-15 % polyacrylamide gels in the presence of SDS, this preparation migrates as a single major band with an apparent molecular weight of 63 kDa. Several faster migrating minor components are also apparent, and may represent fragments of the protective antigen.
Notes:
Binding Ratio: This lot contains 21 ug of FITC bound per mg of PA 63 fragment of anthrax protective antigen, assuming an extinction coefficient at 493 nm of 85,200 M-1 cm- 1 for FITC.
Storage:
This product is packaged aseptically, lyophilized and sealed under vacuum. Store at 2-8 centigrade prior to reconstitution.
Reconstitution:
Anthrax toxin proteins may be reconstituted in sterile distilled water, stored at 4 centigrade and used successfully within a few hours. However, over longer periods of time, there may be a decline in the PA binding. If it is necessary to store this material, reconstitute it at a concentration of 1 mg/ml. 2 Reconstitution with 1 mg/ml BSA may enhance stability and recovery. It is further recommended that the solution is aliquoted and frozen at either -20 centigrade or -70 centigrade. After the protein has been reconstituted as described above, glycerol may be added to 50% if a liquld is desired at freezer temperatures. Storage of material reconstituted in BSA at 4 centigrade for a period of two weeks may be acceptable for some applications.
Warning:
Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. This product is to be used by skilled personnel under the direction of a principal investigator in a laboratory setting only.
2. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
3. Never remove the stopper prior to reconstitution and never work with the product in the powdered form. Always reconstitute it first.
4. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
5. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
6. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.