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Recombinant B. pertussi cyaA Protein

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E. coli(1)
Product Overview:
This product is a recombinant toxin of Bordetella pertussis Tohama strain expressed in E. coli from the structural gene cyaA and its activator gene cyaC and purified by the method of Friedman.
Adenylate cyclase is a toxin produced by a gram-negative coccobacillus, Bordetella pertusis. This toxin penetrates into the animal cells and produces cAMP from ATP in the cytoplasm. It belongs to the RTX family of toxins produced by many gram-negative bacteria.
E. coli
B. pertussis
550 μg/ml in 50 mM Tris-HCl(pH8.0), 6M urea, 150 mM NaCl
Addition of the 10~100 ng/ml adenylate cyclase toxin to medium increased significant levels of cellular cAMP levels in CHO and human monocyte cells as examined by Biotrak cAMP EIA System of GE Healthcare Bioscience.
Molecular Mass:
177 KDa
More than 90% purity by SDS-PAGE (CBB staining)
1) Functional studies on the cAMP- signaling pathway (10~100 ng/ml)
2) Antigen for western blotting and ELISA
3) SDS-PAGE marker as native adenylate cyclase
Shipped with dry-ice and store at -80centigrade.
For research use only, not to be used for human application.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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