Recombinant BEBOV GPDTM fused with His tag was expressed in Insect cells.
Mature, recombinant, His-tagged Bundibugyo glycoprotein minus the transmembrane domain (BEBOV rGPdTM) is supplied as purified protein. BEBOV rGPdTM is produced in Sf9 insect cells using baculovirus for expression and is purified by column chromatography.
100 µg of protein is supplied in PBS (supplemented with 10% glycerol, arginine and glutamic acid)
The theoretical molecular weight of the protein is~73 kDa including the His-tag, without glycosylation. Because of the glycosylated nature of this protein, migration in an SDS-PAGE gel results in a broad band.
2-3 weeks at -20 centigrade, -80 centigrade long term