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Home > Products > Recombinant Proteins > Recombinant Botulinum Neurotoxin Type A Heavy Chain Binding Domain protein, GST-tagged

Recombinant Botulinum Neurotoxin Type A Heavy Chain Binding Domain protein, GST-tagged

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By Species
By Source
E. coli(2)
By Tag
GST(2)
Cat.No.:
BoNT/A-147
Product Overview:
Recombinant Botulinum Neurotoxin Type A Heavy Chain Binding Domain(872-1296 aa) fused with GST tag was expressed in E. coli.
Description:
The protein was recombinantly expressed in E. coli and purified using affinity and anion exchange chromatography.
Source:
E. coli
Species:
C. botulinum
Tag :
GST
Form:
Each vial of recombinant GST Botulinum Neurotoxin Type A Heavy Chain Binding Domain (GST-HccA) contains 50 ug of lyophilized protein. When reconstituted with 140 ul of sterile distilled water, each vial contains 50 pg of GST-HccA in 20 mM HEPES, pH 7.4, 150mM NaCl, 1.25% lactose.
Bio-activity:
GST-HccA is reactive to anti-Botulinum Type A antibodies in a Western Blot. GSTHccA was tested in an ELISA assay using GT1 b and the receptor domain of SV2c. The midpoint of the binding curve of GST-HccA to GT1 b was 10.8 ng. The midpoint of the binding curve to SV2c was 1.5 ng.
Molecular Mass:
The molecular weight of the protein is approximately 77 kDa.
Protein length:
662 amino acids
Purity:
>90%
Notes:
Toxicity: GST-HccA is only a fragment of Botulinum toxin and as such is a non toxic protein.
Storage:
This product is supplied as a lyophilized powder which has been stoppered under vacuum. Reconstitution of the powder should be done with syringe through the rubber stopper to avoid any loss of material. Store lyophilized vials at 4 centigrade. Once dissolved, aliquot and store at -20 centigrade. Refrain from multiple freeze/thaw cycles.
Concentration:
Protein concentration was determined by a modification of the Bradford' method using bovine serum albumin as a standard.
Warning:
Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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