Recombinant Botulinum Neurotoxin Type A Light Chain(1-429 aa) was expressed in E. coli. It also contains residual amino acids from the affinity tag at the N-terminal to give a total 437 aminl acids.
The protein was recombinantly expressed in E. coli and purified using affinity and ion exchange chromatography. The affinity tag has been cleaved prior to quantitation and pakaging.
Each vial of light chain from Botulinum neurotoxin type A when reconstituted with 100 ul water, contains 10ug of protein in 20 mM HEPES, pH 7.4, 1.25 % lactose. The pH 7.4 HEPES buffer was obtained by titrating the free acid form of HEPES with the potassium salt form of HEPES. This minimizes the sodium and chloride concentrations present with the toxin. In order to ensure stability during storage and recovery of the protein 0.05% TWEEN-20 or 1 mg/ml BSA must be included in your reconstitution buffer.
The light chain of Botulinum Neurotoxin is non-toxic and unable to penetrate cells in the absence of the heavy chain. The expression and purification of light chain from a recombinant setting ensures there is no possible contamination with heavy chain or full length intact toxin.
This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store at -20 centigrade. Refrain from multiple freeze/thaw cycles.
Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.