Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Botulinum Neurotoxin Type A Light Chain protein, GST-tagged

Recombinant Botulinum Neurotoxin Type A Light Chain protein, GST-tagged

   Share
"BoNT/A Light Chain" Related Products.
By Species
By Source
E. coli(2)
By Tag
GST(1)
Cat.No.:
BoNT/A-149
Product Overview:
Recombinant Botulinum Neurotoxin Type A Light Chain(1-429 aa) fused with GST tag was expressed in E. coli.
Description:
The protein was recombinantly expressed in E. coli and purified using affinity and ion exchange chromatography. The GST affinity tag has been retained on this protein.
Source:
E. coli
Species:
C. botulinum
Tag :
GST
Form:
Each vial of light chain from Botulinum neurotoxin type A contains 15 ug of protein lyophilized with 180 ul of 20 mM HEPES, pH 7.4, 1.25 % lactose. The pH 7.4 HEPES buffer was obtained by titrating the free acid form of HEPES with the potassium salt form of HEPES. This minimizes the sodium and chloride concentrations present with the toxin. In order to ensure stability during storage and recovery of the protein 0.05% TWEEN-20 or 1 mg/ml BSA must be included in your reconstitution buffer.
Bio-activity:
The recombinant Light Chain- GST fusion has been tested for activity in a FRET based assay. A standard curve was generated using SNAPtide Unquenched Calibration Peptide. When using SNAPtide substrate, cleavage can be easily detected at 5 nanomolar concentration of protein.
Molecular Mass:
The light chain A fragment contains amino acids 1-429 of the full length Botulinum Neurotoxin Type A for a molecular weight of approximately 50 kDa. The GST tag is 26 kDa. The total molecular weight of the fusion protein based on sequence is 76.389 kDa.
Purity:
>80%
Notes:
The light chain of Botulinum Neurotoxin is non-toxic and unable to penetrate cells in the absence of the heavy chain. The expression and purification of light chain from a recombinant setting ensures there is no possible contamination with heavy chain or full length intact toxin.
Storage:
This product is supplied as a lyophilized powder which has been stoppered under vacuum. Reconstitution of the powder should be done with syringe through the rubber stopper to avoid any loss of material. Store lyophilized vials at 4 centigrade. Once dissolved, aliquot and store at -20 centigrade. Refrain from multiple freeze/thaw cycles.
Warning:
Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.