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Home > Products > Recombinant Proteins > Recombinant Botulinum Neurotoxin Type B Light Chain protein

Recombinant Botulinum Neurotoxin Type B Light Chain protein

"BoNT/B Light Chain" Related Products.
By Species
By Source
E. coli(1)
Product Overview:
The light chain type B fragment contains amino acids 1-436 of the full length botulinum neurotoxin type B. It also contains eight residual amino acids from the affinity tag at the N - terminus to give a total length of 444 amino acids.
The protein was recombinantly expressed in E. coli and purified using affinity and anion exchange chromatography. The affinity tag has subsequently been cleaved from the protein prior to quantitation and packaging.
E. coli
C. botulinum
Each vial contains 10 ug of light chain from botulinum neurotoxin type B. When reconstituted with 0.1 ml of water, the concentration of the buffer is 20 mM HEPES, pH 7.4, 1.25% lactose. In order to ensure stability during storage of the protein, 0.05% TWEEIN-20 or 1 mg/ml BSA must be included in the reconstitution buffer. Handle the product gently, do not vortex.
The protein has been tested for activity in a FRET endopeptidase assay. Significant cleavage of the fluorogenic peptide substrate VAMPtide was seen with 2.5, 5, and 10 nM enzyme.
Molecular Mass:
The molecular weight of the protein has been determined to be 51.210 kDa based on electorspray-MS analysis.
Protein length:
444 amino acids
Toxicity: The light chains of the botulinum neurotoxins are non-toxic and unable to penetrate cells in the absence of the heavy chain (binding and translocation domains). The expression and purification of light chain from a recombinant setting ensures there is no possible contamination with heavy chain or full length intact toxin.
This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store the product at -20 centigrade. Refrain from multiple freeze/thaw cycles.
Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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