Recombinant Botulinum Neurotoxin Type F Light Chain(1 - 428 aa) fused with GST tag was expressed in E. coli.
The protein was recombinantly expressed in E. coli. The GST affinity tag has been cleaved from the protein prior to quantitation and packaging.
Each vial of contain 10 ug Light Chain from Botulin um Neurotoxin Type F (BoNT/F). When reconstituted with 100 ul of water, the concentration buffer is 20 mM HEPES, pH 7.4 + 1.25% lactose. In order to ensure stability during storage of the protein, 0.05% TWEEN-20 or 1 mg/ml BSA must be included in the reconstitution buffer. Handle the product gently, do not vortex.
428 amino acids
The light chain of Botulinum Neurotoxin is non-toxic and unable to penetrate cells in the absence of the heavy chain. The expression and purification of light chain from a recombinant setting ensures there is no possible contamination with heavy chain or full length intact toxin.
This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store the product at -20 centigrade. Refrain from multiple freezelthaw cycles.
Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.