Recombinant Canine BMPR1A Protein, Fc-tagged
Recombinant canine BMPR1A (NP_001138622.1) (Met1-Arg152) was expressed with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to inhibit rhBMP4 induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.5-2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
The recombinant canine BMPR1A consists 370 amino acids and predicts a molecular mass of 41.2 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>(82.9 + 16.4) % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.