Recombinant Canine IL12B(Ile23-Ser329) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to compete with biotinylated canine IL-12/IL-23 p40 for binding with immobilized rhIL-12 R beta 1/Fc Chimera in a functional ELISA assay.
Predicted Molecular Mass: 36.3 (monomer) kDaSDS-PAGE: 43 kDa, reducing conditions
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS.