Recombinant mature form of canine IL33 (O97863) (Ser 110-Ser 263) was expressed, with an initial Met at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile 5mM EDTA, PBS, pH 7.51, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc, The EC50 of human IL1R4-Fc is 0.25-0.66 μg/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cel
The recombinant canine IL33 consists of 155 amino acids and has a calculated molecular mass of 17.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19KDa band.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.